1632 ratings. The interest toward this extraction technique is increasing because it presents several advantages toward classical extraction techniques like increased extraction yields, higher quality of extracts, less extraction time, and decreased solvent consumption (Xi, 2015). The results showed significant histological improvement and a reduction in NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells), TNF , COX2 an iNOS levels, all of which support the anti-inflammatory effect of berberine (Domitrovi et al., 2013). (1980). Berberine and sanguinarine quantitation in, Pressurized liquid extraction as a green approach in food and herbal plants extraction: a review. Berberine has also been used in eye drops for eyes or gels meant for use in the mouth for canker sores. Tiwary J. K., Ballabha R., Tiwari P. (2010). El Beyrouthy M., Arnold N., Delelis-Dusollier A., Dupont F. (2008). (2010). Biomed. Berberine ameliorates experimental autoimmune neuritis by suppressing both cellular and humoral immunity. The immunomodulatory effect of berberine was demonstrated in many experimental and clinical contexts. Determination of berberine content in the stem extracts of, Etude sur les plantes medicinales et toxiques d'Afrique equatoriale. Ogbonna D. N., Sokari T. G., Agomuoh A. OREGON GRAPE - Uses, Side Effects, and More - WebMD Thermally treated berberine-loaded SA/PVA/PEO electrospun microfiber membranes for antibacterial wound dressings. Found wild in the western states, this species is uncommon in New England, mainly recorded as garden escapes in Vermont. If you're searching for the right natural product to clear all acne complications, this berberis aquifolium is your ideal choice. Additives like formic acid, acetic acid, and ammonium acetate were added in different concentrations as follows: 0.1, 0.5, 1, and 2% for formic acid, 0.1, 0.5, 1, and 2% for acetic acid and 0.0001, 0.001, 0.01 mol/L for ammonium acetate. Berberine may have a lipid-lowering effect. Different species of berberine-rich plants have multiple pharmacological and therapeutic actions, such as antioxidant and immunomodulatory effects, protective action on the cardiovascular system, liver and kidney, endothelial relaxation, regulator on glucose metabolism and atherosclerosis, which can all be explained by the presence of berberine as well as other phyto constituents (when dealing with berberine-containing plant extracts). The value of plants used in traditional medicine for drug discovery. Compared with conventional methods, PLE increases the extraction yield, decreases time and solvent consumption, and protects sensitive compounds. The oldest evidence of using barberry fruit (Berberis vulgaris) as a blood purifying agent was written on the clay tablets in the library of Assyrian emperor Asurbanipal during 650 BC (Karimov, 1993). B., Travih V. A. Featured Creature Edition: December 2018, Crater Lake National Park, Oregon Caves National Monument & Preserve, Redwood National and State Parks, Whiskeytown National Recreation Area, Download the official NPS app before your next visit, https://oregonencyclopedia.org/articles/oregon_grape/#.XA_5RnRKi70, http://www.metrofieldguide.com/wildlife-plants-oregon-grape/#, NPS Klamath Inventory & Monitoring Network, oregon caves national monument & preserve, Oregon Caves National Monument & Preserve. Rivera Nez D., Obon de Castro C. (1996). Some of the plants that contain berberine includeCoptis chinensis (Huanglian), Rhizoma coptidis, Hydrastis canadensis (goldenseal), Berberis aquifolium (Oregon grape), Berberis vulgaris (barberry), Berberis aristata (tree turmeric), Tinospora cordifolia, Coptidis rhizome, Arcangelisia flava, and Cortex phellodendri. Berberine improves glucose metabolism through induction of glycolysis, Efficacy of berberine in patients with type 2 diabetes mellitus. Wang Y., Yi X., Ghanam K., Zhang S., Zhao T., Zhu X. (1985). Oregon Grape - U.S. National Park Service Esseily F., El Ezzy M., Gali-Muhtasib H., Safi S., Esseily J., Diab-Assaf M., et al. However, there are some traditional uses that have not yet been completely elucidated, and further studies are needed. The .gov means its official. (2011). Other studies performed on animal models with CCl4induced hepatotoxicity demonstrated the same effect (Othman et al., 2014). In addition, berberine has a favorable influence on the lipid profile, unlike metformin, which has barely any effect (Yin et al., 2008b). A comparative study on the anti-inflammatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the roots of Turkish, Isolation and quantitative determination of berberine and coptisine from tubers of. The bark, root, and root-like stem (rhizome) are used to make medicine. Okunade A. L., Hufford C. D., Richardson M. D., Peterson J. R., Clar A. M. (1994). The decrease in transaminase levels supports the hypothesis according to which berberine helps maintain the integrity of the hepatocellular membrane (Domitrovi et al., 2011). Received 2017 Dec 21; Accepted 2018 May 9. Edible, Medicinal Berberis aquifolium | Oregongrape | Edible and Medicinal Uses Gallery botanic View photos of the edible and medicinal plant Berberis aquifolium [Mahonia aquifolium] (Oregongrape), profiled in Herbal Medicine: Trends and Traditions and Medicinal Plants of the Western Mountain States. Addition of berberine to 5-aminosalicylic acid for treatment of dextran sulfate sodium-induced chronic colitis in C57BL/6 Mice. Misra P. S., Bhakuni D. S., Sharma V. N., Kaul K. N. (1961). Yu C., Tan S., Zhou C., Zhu C., Kang X., Liu S., et al.. (2016). (2006). In Yunani medicine, Berberis asiatica has multiple uses, such as for the treatment of asthma, eye sores, jaundice, skin pigmentation, and toothache, as well as for favoring the elimination of inflammation and swelling, and for drying ulcers (Kirtikar and Basu, 1998). unintentionally); has become naturalized. Liang Y, Xu X, Yin M, et al. High-density lipoprotein (HDL) cholesterol, considered "good" cholesterol, and sexhormonebinding globulin levels were higher in the berberine group. Bushra I., Kishwar S., Qureshi R. A., Saddiqa M. (2000). Berberine inhibits this kinase, thereby exercising its cardioprotective effect (Park et al., 2014). Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya. However, these forms might not be available for purchase in many places. Kiryakov H. G., Daskalova E., Georgieva A., Kuzmanov B., Evstatieva L. (1982a). Andola H. C., Rawal R. S., Rawat M. S. M., Bhatt I. D., Purohit V. K. (2010c). Berberis Aquifolium uses are many ranging from treating skin issues, eye soreness, joint pains, headache acidity etc. Prepared by Sonya Daw Zhao X., Zhang J., Tong N., Chen Y., Luo Y. Les medicaments des pygmees Baka du Cameroun: moeurs therapeutiques, maladies et inventaire des plantes medicinales, Ethno medicines used for treatment of prostatic disease in Foumban, Cameroon, Insecticides and ethnomedicine of HIV/AIDS at Tokombere (Far North Cameroon), Plants and treatment of prostatic diseases in Foumban (West Region, Cameroon). O.R.S.T.O.M. Antimicrobial activity of crude extracts from. This beneficial effect could partially be attributed to the inhibition of NF-kB and the reduction in JAK2 phosphorylation (through the influence on the JAK/STAT pathway) by both 5-ASA and berberine (Li et al., 2015; Figure Figure33). Tall Oregon Grape, Berberis aquifolium - Calscape Ethnobotanical survey of the uses Annonaceae around mount Cameroon. Chhetri D. R., Parajuli P., Subba G. C. (2005). V. Muramine and berberine from. Polymers (Basel). Two other commonly used techniques in berberine quantification are thin layer chromatography (TLC) and high performance thin layer chromatography (HTPLC). Bapna S., Choudhary P. K., Ramaiya M., Chowdhary A. We depend on JAK/STAT signaling is associated with cardiac dysfunction during ischemia and reperfusion. Guo J, Chen H, Zhang X, et al. Nat. Nowadays, a significant number of dietary supplements based on plants containing berberine (Kataoka et al., 2008) are used for reducing fever, common cold, respiratory infections, and influenza (Fabricant and Farnsworth, 2001). a sighting. Berberine in cardiovascular and metabolic diseases: From mechanisms to therapeutics. Kiryakov H. G., Iskrenova E., Daskalova E., Kuzmanov B., Evstatieva L. (1982b). (2011). Along with extraction temperature, the choice of solvents is considered a critical step in berberine extraction as well (Figure (Figure2).2). Homeopathic Remedies for Clear and Glowing Skin - homeopathy It can be utilized in every one of the situations where the skin is dim or has scars because of skin inflammation or some other conditions. Alkaloids of, Alkaloids of berberidaceous plants. official website and that any information you provide is encrypted Additionally, the authors recorded higher pro-oxidant and lower antioxidant levels in the intervention group. Part LXXXVIII. Joshi and Kanaki (2013) quantified berberine in Rasayana churna samples in the range of 220 g/mL, the interference with other compounds being avoided by the specific isolation of the alkaloid fraction (Joshi and Kanaki, 2013). Several researches found that berberine is widely distributed in the barks, roots, and stems of plants, nevertheless, bark and roots are richer in berberine compared to other plant parts (Andola et al., 2010a,b). The principles behind these methods consist of the interconversion reaction between the protoberberine salt and the base. (1974). The pellets melt in your mouth and can be taken on the go, no food or water needed. Koni M. Z., Kremer D., Schhly W., Brantner A., Karlovi K., Kalodera Z. Phylogenetic systematics of Berberidaceae and Ranunculales (Magnoliidae). donations to help keep this site free and up to date for For that reason, its important to not take it along with certain medications unless directed to do so. Herb-drug interactions: what the science says. In the fall, the leaves can take on red and burgundy hues, adding to the plants overall ornamental color palette through the course of a season. Unrelated to true grapes in the family Vitaceae, Oregon grape is an evergreen shrub, typically growing in the forest understory. For example, Rojsanga and Gritsanapan (2005) used maceration process to extract 100 g of C. fenestratum plant material with a total volume of 3,200 mL solvent (80% ethanol) over a period of 416 h. Furthermore, in a different study, Rojsanga et al. (2015). Ethnobotany and phytomedicine of the upper Nyong valley forest in Cameroon. Karimov A., Meliboev S., Olimov V., Shakirov R. (1993). Berberine: Uses, Benefits, Side Effects, Dosage, Precautions The patients received 300 mg berberine/day for 24 months, with 2-week interruptions every 5 months. It is resistant to summer drought, tolerates poor soils, and does not create excessive leaf litter. Berberine may work similarly to those drugs and therefore could cause an over effect. Potent antiprotozoal activity of a novel semi-synthetic berberine derivative. 2021;133:110984. doi: 10.1016/j.biopha.2020.110984. (2010). Khamidov I. I., Aripova S. F., Telezhenetskaya M. V., Karimov A., Dzhenberov I. Uniyal S. K., Singh K. N., Jamwal P., Lal B. Berberis aquifolium in Global Plants on JSTOR 1234). Uttarakhand, India (Psychomedicine Medice). 2013;13:475-82. doi:10.1517/14712598.2013.776037, Lan J, Zhao Y, Dong F, Yan Z, Zheng W, Fan J, Sun G. Meta-analysis of the effect and safety of berberine in the treatment of type 2 diabetes mellitus, hyperlipemia and hypertension. 2019;9:1923-1951. doi: 10.7150/thno.30787. Alkaloids of Papaveraceae. Berberine derivatives as antileishmanial drugs. (2014). Shahid M., Rahim T., Shahzad A., Latif T. A., Fatma T., Rashid M., et al. Bele M. Y., Focho D. A., Egbe E. A., Chuyong B. G. (2011). Glossary of Indian Medicinal Plants (Including the Supplement), Berberine: metabolic and cardiovascular effects in preclinical and clinical trials, The History and Folklore of North American Wildflowers. Big differences were observed in the extraction time, PLE requiring only 30 min for a single sample extraction compared to 2 h for multiple extraction techniques or 6 h for Soxhlet extraction (Schieffer and Pfeiffer, 2001). [14], In some areas outside its native range, M. aquifolium has been classified as an invasive exotic species that may displace native vegetation. In California and the Pacific Northwest, one of the most common speciesand Oregon's state floweris Berberis aquifolium (also called tall Oregon . The developed method was reproducible, validated, precise, and specific for berberine quantification (with a concentration range between 0.2 and 150 g/mL; Shigwan et al., 2013). Additionally, many clinical and experimental studies suggest that berberine has several pharmacological properties, such as immunomodulatory, antioxidative, cardioprotective, hepatoprotective, and renoprotective effects. Alkaloids of, Preliminary list of medicinal and economic kansas plants, with their reputed therapeutic properties. ], Some Indigenous peoples of the Northwest Plateau use Oregon grape for indigestion. (2014). The effect of berberine was studied in combination with 5-ASA (5-aminosalicylic acid) vs. 5-ASA alone using an experimental animal model with DSS (dextran sulfate sodium)-induced colitis. This is because it may have an effect on a fetus or a newborn. (2012) demonstrated that temperature represent a crucial factor in both extraction and drying treatments prior extraction. The Nlakapamux People of British Columbia applied it to their basketry. Berberine and neurodegeneration: a review of literature, Illustrated Book of Korean Medicinal Herbs. However, it is important to discuss its use with a healthcare provider first. Berberis aquifolium is resistant to infection by Puccinia graminis. Supplements should be stored in a cool, dry place and out of the reach of children. Synthesis of linked berberine dimers and their remarkably enhanced DNA-binding affinities. Discover thousands of Berber - an overview | ScienceDirect Topics For example, Shigwan et al. (1989). Pharmacokinetic studies of novel berberine derivatives with ultra-performance liquid chromatographytandem mass spectrometry. Super critical fluid extraction is another environmentally friendly efficient technique used in phytochemical extraction. Berberine paired with a medication was also more effective than the medication used alone. Lately, MAE was used as a green and cost-effective alternative to conventional methods. Oregon grape is used for scaly, itchy skin ( psoriasis ), eczema ( atopic dermatitis ),. Combination of simvastatin with berberine improves the lipid-lowering efficacy. Ali M., Shah S. Z., Khan M. S., Naz M. F. R., Zafar A. Ethnopharmacological application of medicinal plants to cure skin diseases and in folk cosmetics among the tribal communities of North-West Frontier Province, Pakistan. The flowers ripen into round, dusty blue-black berries, resembling grapes, by early fall. Please review supplement labels with a healthcare provider to look for potential interactions with foods, other supplements, and medications. Berberis aquifolium is the state flower of Oregon. Oral Med. An investigation of the alkaloids of Berberis thunbergii. All images and text Hamonniere M., Leboeuf A., Paris R. R. (1975). (1997b). Berberine is largely considered to be safe. A small, double-blind, placebo-controlled trial was done on 144 White people with high cholesterol but no other risk factors for heart disease. Liu W. H., Hei Z. Q., Nie H., Tang F. T., Huang H. Q., Li X. J., et al.. (2008a). The first group was treated with berberine (1,000 mg/day), the second with simvastatin (20 mg/day) and the third with a combination of berberine and simvastatin. ACAT1 is a ubiquitous enzyme, while ACAT2 can be found only in hepatic cells and enterocytes. Dzhalilov D. R., Goryaev M. I., Kruglykhina G. K. (1963). Pnkov K., Urbanov J., Musil P., Tborsk E., Gregorov J. Under normal conditions, the body maintains a balance between the antioxidant and pro-oxidant agents (reactive oxygen speciesROS and reactive nitrogen speciesRNS; Rahal et al., 2014). Show Berberine structure (according to ChemSpider database). The leathery leaves resemble those of holly. Each of the six stamens, terminates in two spreading branches. A., Beckstrom-Sternberg S. M. (1994). (2006). Thank you, {{form.email}}, for signing up. Experimental autoimmune neuritis is an experimental animal model equivalent to the Guillain-Barre syndrome in humans. 2012;166:99-105. doi:10.1530/EJE-11-0616, Sahibzada MUK, Sadiq A, Faidah HS, et al. For these reasons, berberine is being looked at to use as a supplement for several conditions. It is valued for its striking foliage and flowers, which often appear before those of other shrubs. Note: when native and non-native 1. la Rech. A. Comparative studies of berberine concentration contained in different species of the same genus have been reported, e.g., higher berberine content in B. asiatica (4.3%) in comparison to B. lycium (4.0%), and B. aristata (3.8%). Coptidis rhizoma (rhizomes of Coptis chinensis), another plant which contains berberine, is a famous herb very frequently used in traditional Chinese medicine for the elimination of toxins, damp-heat syndromes, purge fire, and to clear heat in the liver (Tang et al., 2009). Your help is appreciated. This article was published under the frame of the internal grant no. Issues New Crop. Targeting Aberrant Expression of STAT3 and AP-1 Oncogenic - PubMed XXVIII. Berberine inhibits dyslipidemia in C57BL/6 mice with lipopolysaccharide induced inflammation. Form: Upright or erect. In this case, 0.34% phosphoric acid concentration was considered optimal. Simultaneous determination of eight alkaloids in rat plasma by UHPLC-MS/MS after oral administration of. However, the authors note that berberine didnt work in all cases. Xu K., He G., Qin J., Cheng X., He H., Zhang D., et al.. (2017). Ransohoff R. M., Hafler D. A., Lucchinetti C. F. (2015). Legal status:Available over the counter (OTC), Suggested dose: 500 milligrams (mg), 2 or 3 times a day, Safety considerations: May overlap with the effectiveness of some prescription medications and should therefore be used with caution. (1989). Khamidov I., Karimov A. K., Telezhenetskaya M. V., Tashkhodzhaev B. In Meyler's Side Effects of Drugs (Sixteenth Edition), 2016. Pretreatment with berberine significantly reduced both functional hepatic tests and histological damage (inflammatory cellular infiltrate, hepatocyte necrosis; Zhao et al., 2012). Berberine extracts and decoctions are traditionally used for their activities against a variety of microorganisms including bacteria, viruses, fungi, protozoa, helminthes, in Ayurvedic, Chinese, and Middle-Eastern folk medicines (Tang et al., 2009; Gu et al., 2010). A decrease in the extraction time (39.81 min) was obtained by Chang (2013). Purification and characterization of the berberine bridge enzyme from, Alkaloids of the Papaveraceae. Berberine is also used in traditional Chinese medicine. tritici; the primary host of Pst being wheat). UV-Vis spectrophotometry can be considered as one of the most rapid detection methods for berberine quantitative analysis from plant extracts. Conservation studies on four medicinal taxa of Southern Nigeria. (2004). Inclusion in an NLM database does not imply endorsement of, or agreement with, Deng Y., Liao Q., Li S., Bi K., Pan B., Xie Z. It sets off a chain of events that reduces the bodys ability to uptake long-chain fatty acids. AMPK is an important enzyme playing an essential role in cellular metabolism and offering protection in ischemic conditions by adjusting the carbohydrate and lipid metabolism, the function of cell organelles (mitochondria, endoplasmic reticulum) and the apoptosis (Zaha et al., 2016). Mahonia aquifolium, the Oregon grape or holly-leaved barberry, is a species of flowering plant in the family Berberidaceae, native to western North America. A. Marroqun, Jorge S., & Joseph E. Laferrire. Berberis aquifolium Ethnobot, The philosophy of African medical practice. Also covers Berberis aquifolium - Plant Finder - Missouri Botanical Garden Alkaloids From Iraqi Species of Papaveraceae. Berberine is also used in traditional Chinese medicine . 2021;42:101287. doi:10.1016/j.ctcp.2020.101287, Wu XK, Wang YY, Liu JP, et al; Reproductive and Developmental Network in Chinese Medicine. Pfoze N. L., Myrboh B., Kumar Y., Rohman R. (2014). Distribution of certain poppy-fumaria alkaloids and a possible link with the incidence of glaucoma, Common medicinal folk recipes of District Buner, NWFP, Pakistan. [citation needed], Mahonia aquifolium is a native plant in the North American West from Southeast Alaska to Northern California, and eastern Alberta to central New Mexico, often occurring in the understory of Douglas-fir forests (although other forest types contain the species) and in brushlands in the Cascades, Rockies, and northern Sierra Nevada. After extraction and purification, the separation and quantification of berberine are commonly resolved by chromatographic methods. It does show promise in treating some conditions, most commonly in combination with medications. They come in an easy dispensing tube of approx. Anthropogenic (man-made or disturbed habitats), forest edges, Occurs only in non-wetlands. Wu X., Li Y., Wang Q., Li W., Feng Y. Plants of this genus are used against inflammation, infectious diseases, diabetes, constipation, and other pathologies (Singh A. et al., 2010).The oldest evidence of using barberry fruit (Berberis vulgaris) as a . Berberis Aquifolium : Buy Berberis Aquifolium Products Online in - 1mg (2015). Baharvand-Ahmadi B., Bahmani M., Tajeddini P., Naghdi N., Rafieian-Kopaei M. (2016). (2008). ADD. This enzyme can attach itself to LDL receptors, leading to a decrease in LDL metabolization and an increase in its blood level (Xiao et al., 2012). Soedin. Chem. The alkaloids of fumariaceous plants. (Wetland indicator code: Ethnopharmacological survey of Annonaceae medicinal plants used to treat malaria in four areas of Cameroon. It is known by a large variety of different common names including Oregon hollygrape, Oregon grapeholly or Oregon grape. GSK3-Dependent inhibition of AMPK potentiates activation of neutrophils and macrophages and enhances severity of acute lung injury. 2020;19:71-80. doi:10.1007/s40200-019-00478-z, Chang W, Chen L, Hatch GM. The Useful Plants of West Tropical Africa. (1996b). The magnitude of the beneficial effect was in direct proportion with the plasma concentration of berberine (Zeng, 1999). Chakravarti K. K., Dhar D. C., Siddiqui S. (1950). image, please click it to see who you will need to contact. Traditional uses of berberine-containing species. Analysis of berberine content using HPTLC fingerprinting of root and bark of three Himalayan, Screening of plants used in Argentine folk medicine for antimicrobial activity. (2009). Protection against myocardial ischemia/reperfusion injury in TLR4-deficient mice is mediated through a phosphoinositide 3-kinase-dependent mechanism. Stermitz F. R., Stermitz J. R., Zanoni T. A., Gillespie J. Combo Pack of Dr Willmar Schwabe India Berberis Aquifolium Mother Tincture Q 30ml & SBL Berberis Aquifolium Gel 25gm. However, even if supplements are third-party tested, that doesnt mean they are necessarily safe for all or effective in general. Marek R., Seckrov P., Hulov D., Marek J., Dostl J., Sklenr V. (2003). Botanists call this configuration pinnate, from the Latin word pinnatus, meaning feathered. Berberis Aquifolium | Boiron USA Long ago, American Indian tribes in the Pacific Northwest used Oregon grape for many ailments, including stomach problems, hemorrhages, tuberculosis, and arthritis. Antimalarial activities of some selected traditional herbs from Southeastern Nigeria against. Uchiyama T., Kamikawa H., Ogita Z. Additionally, it has been reported the use of decoction of Indian barberry and Emblic myrobalan mixed with honey in the cure of urinary disorders as painful micturition (Kirtikar and Basu, 1998). evidence (herbarium specimen, photograph). Its thought that berberine might be helpful as a complementary treatment for diabetes. The Berberis genus also goes by Mahonia, depending on taxonomy. Evaluation of antioxidant activities and phenolic content of, Treat jaundice, hepatitis A, B, C, and D, conjunctivitis, leishmaniasis, medicine for cuts and infected wounds, sores and ulcers, antipyretic for various fevers, tuberculosis, vomiting of blood, urinary tract infections, treatment of fatigue, rheumatism, treat malaria symptoms, aches, wounds, boils, vomiting, yellow bitter, chills, sore, spleen in children and body pains, skin ailments, intercostal pain and to promote conception, intestinal worms, intestinal spasms, malaria and sexual asthenia, treat coughs and wounds; rickettsia fever, treat of sleeping sickness and dysentery, hemostatic and rickettsia, treat yellow fever and typhoid fever, treat diabetes, treat syphilis, and other infectious diseases, poliomyelitis, treat hypertension, treat HIV and prostate cancer, Treat cuts, antiseptic to treat sores, stomach ulcers, leprosy and ophthalmia, treatment of skin infections and sores, treat jaundice, and treat fever including malaria and to promote wound healing, against intestinal problems, Treat wounds, ulcers, leprosy, rheumatism, stomach and gall-bladder problems, eye diseases, for conception, diarrhea, malaria, fevers and sleeping disorders, Skin conditions, treat eczema, acne, conjunctivitis and herpes, alleviate the symptoms of psoriasis, treat diarrhea and in higher doses to treat constipation, improvement of blood flow to the liver, stimulate intestinal secretions and bile flow, treat jaundice, hepatitis, cirrhosis and general digestive problems, treatment of gall bladder disease, hemorrhages and a few forms of cancer, fungal infections, dysentery, anti-inflammatory properties, stomach problems, sore womb following childbirth and/or menstruation, Treat allergies, metabolic disorders, ophthalmia, and other eye diseases, treat bleeding piles, anti-osteoporosis, treat skin diseases, menorrhagia, fever, diarrhea, dysentery, cholera, jaundice, ear and urinary tract infections, anti-bacterial, anti-fungal, anti-inflammatory, analgesic, anti-pyretic, jaundice, piles, malaria, laxative, anti-scorbutic, anti-diabetic, and anti-hepatopathic, Jaundice, diabetes mellitus, wound healing, asthma; drying unhealthy ulcers, anti-inflammatory, swelling, treat pneumococcal infections, eye (conjunctivitis) and ear diseases, rheumatism, fever, stomach disorders, skin disease (hyperpigmentation), malarial fever, laxative, teeth problems (toothache), and headache, Treat skin disease, jaundice, rheumatism, affection of eyes (household treatment for conjunctivitis, ophthalmic, bleeding piles), ulcers, skin diseases, enlarged liver and spleen, Antipyrectic, anti-inflammatory, treat stomach pains, indigestion, and colitis, Antipyretic, treat diabetes, bone fractures, rheumatism, radiculitis, heart pain, stomach aches, kidney stones, tuberculosis, chest pain, headaches, constipation, and wound, Antipyretic, treat gastroenteritis, sore throats, and conjunctivitis, Antipyretic, cold and complications during post-natal period, Treat rheumatic and neuralgic diseases, anti-inflammatory, treat arthritis and muscular pain, Treat eye diseases, febrifuge, jaundice, diarrhoea, menorrhagia, piles, backache, dysentery, earache, fracture, eye ache, pimples, boils, wound healing, cough and throat pain, intestinal colic, diabetes, throat pain, scabies, bone fractures, sun blindness, against stomachache and intestinal problems, Febrifuge, anti-inflammatory and treat diarrhea, Heart tonic, treat neurasthenia, antipyretic, antidiarrheal, treat rheumatism, eye diseases and wounds of the mouth, jaundice, stomach aches, back pain and arthralgia, Treat malarial fever, diarrhea, conjunctivitis, and jaundice, Diuretic, treat jaundice, intestinal disorders, eye diseases, oxytocic and throat ache, stomach problems and ulcers, Antimicrobial for skin disease, jaundice, affection of eyes, treat menorrhagia, diarrhea, and rheumatism, Treating fever, jaundice, nausea, eye disorders and skin problems, tonic, Antiarrhythmic, sedative, anticancer, heal internal injuries, remove kidney stones, treat sore throat and fever, Menstrual cramps, relieve the pain of childbirth, promote prompt delivery, treat colics, cramps, hysteria, rheumatism, uterine stimulant, inducer of menstruation, and antispasmodic, Antispasmodic, diuretic, emetic, expectorant, treat diarrhea, dropsy, gravel and urinary problems, emetic, expectorant, treat sores, ulcers and inflamed parts, Anticancer, febrifuge, antiodontalgic, treat testicular swelling and arthritic pain, Diuretic, demulcent, treat dysentery and inflammations of the eyes, Antitussive, astringent, febrifuge, stomachic and tonic, treat of fever in influenza, acute bronchitis, whooping cough, indigestion, acute gastro-enteritis, tooth abscess, pain in the bones, muscles and traumatic injuries, and antirheumatic, Regulate menstruation, promote the circulation of blood, treat amenorrhea, difficult labor and retention of dead fetus or placenta, Tonic, antiperiodic, anti-spasmodic, anti-inflammatory, antiarthritic, anti-allergic, anti-diabetic, improve the immune system, antistress, anti-leprotic and anti-malarial activities, Anthydropic, cathartic, diaphoretic, diuretic, demulcent, emetic, purgative, treat jaundice, skin ailments, colds, colics and wounds, Analgesic, antispasmodic, sedative, treat warts, cold sores, cutaneous affections, skin diseases, itches, treat cataracts, treat dropsy, jaundice, treat chronic skin diseases, expectorant, treat coughs and chest complaints, demulcent, emetic, expectorant, laxative and antidote to snake poisoning, Treat respiratory ailments as asthma, cough, bronchitis and pneumonia, Treat skin conditions (ulcers and eruptions) and respiratory tract infections (bronchistis and tuberculosis), Treat ophthalmic diseases (remove films from the cornea of the eye), mild sedative, antispasmodic, relaxing the muscles of the bronchial tubes and intestines, treat warts, alterative, anodyne, antispasmodic, cholagogue, diaphoretic, diuretic, hydrogogue, narcotic, purgative, treat bronchitis, whooping cough, asthma, jaundice, gallstones and gallbladder pains, anticancer, analgesic, treat stomach ulcer, treat get rid of warts, ringworm and corns, Anodyne, antibacterial, antispasmodic, hallucinogenic, calm the nerves, sedative for insomnia, CNS stimulant, painkiller, treat painful menstruation, lowering the blood pressure, traumatic injury and lumbago, Treat memory dysfunction, treat gastric, duodenal ulcer, cardiac arrhythmia disease, rheumatism and dysmenorrhea, Sedative, diuretic, relieve pain, relax spasms, promote perspiration, treat nervous tension, anxiety, insomnia, urinary incontinence (especially in children), narcotic, relieve toothache, antispasmodic, analgesic and suppress the flow of milk in lactating women, Reduce warts, antitusive, treat CNS disturbances, sedative, cooling, and mild laxative, Analgesic, antioedemic, carminative, depurative, diuretic, treat insect bites, and ringworm, Treat some skin diseases and inflammation, Sudorific, diuretic, expectorant and ophthalmia, Ailments in the elderly and children, mild pain reliever, treat irritable coughs, reduce nervous over-activity, anodyne, emollient, emmenagogue, expectorant, hypnotic, slightly narcotic, sedative, treat bronchial complaints and coughs, insomnia, poor digestion, nervous digestive disorders and minor painful conditions, treat jaundice, fevers, and anticancer, Treat dermatologic diseases, anti-infective, diuretic, sedative, and antitussive, Control of bacterial and viral infections, relax spasms, lower fevers, stimulate the circulation, treat diabetes mellitus, analgesic, locally anaesthetic, antibacterial, antipyretic, bitter, blood tonic, carminative, cholagogue, digestive, sedative, stomachic, vasodilator, treat diarrhoea, acute enteritis and dysentery, treat insomnia, fidget, delirium due to high fever, leukaemia and otitis media, treat conjunctivitis, skin problems (acne, boils, abscesses and burns whilst), mouth, tongue ulcers, swollen gums, and toothache, Control of bacterial and viral infections, relax spasms, lower fevers, stimulate the circulation, locally analgesic and anaesthetic, anti-inflammatory, stomachic, treat conjunctivitis, intestinal catarrh, dysentery, enteritis, high fevers, inflamed mouth and tongue, Control of bacterial and viral infections, relaxes spasms, lowers fevers and stimulate the circulation, locally analgesic, anaesthetic, ophthalmic and pectoral diseases, effective antibacterial, treat dysentery, Treat disorders of the digestive system and mucous membranes, treat constipation, antiperiodic, antiseptic, astringent, cholagogue, diuretic, laxative, stomachic, tonic, treat disorders affecting the ears, eyes, throat, nose, stomach, intestines, and vagina, Treat mouth ulcers, stomach ulcers, colds, jaundice, treat piles, and digestive disorders, Treat gastroenteritis, abdominal pain and diarrhea, antiinflammator, immunostimulator and treat cancer (antitumor activities), Act strongly on the kidneys, detoxicant for hot damp conditions, treat meningitis, conjunctivitis, antibacterial, antirheumatic, aphrodisiac, bitter stomachic, cholagogue, diuretic, expectorant, febrifuge, hypoglycaemic, treat ophtalmia, skin, vasodilator and tonic, treat acute diarrhoea, dysentery, jaundice, vaginal infections (with, African whitewood, african yellow wood Epfou, Py, Nfol, Poyo, Chutro, rasanjan (Nep); marpyashi (Newa); daruharidra, darbi (Sans), Patagonian barberry, magellan barberry, calafate, Creeping mahonia, creeping Oregon grape, creeping barberry, or prostrate barberry, Nandina, heavenly bamboo or sacred bamboo, White prickly poppy, Bluestem pricklypoppy, Plume poppy, tree poppy, tree celandine, parrotweed, sea oxeye daisy, john crow bush, Red Horned Poppy, Grand-flowered Horned Poppy, Mixing 100 l sample with 3% formic acid in acetonitrile (200 l), Residue solubilization: in 100 l of 20% methanol, Evaporation of 10 ul IS in the working tube.
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